6533b7d2fe1ef96bd125ec4a
RESEARCH PRODUCT
Control of the electronic energy transfer pathway between two single fluorophores by dual pulse excitation.
Thomas BaschéBurkhard FückelKlaus MüllenFabian NoldeGerald Hinzesubject
Physics::Biological Physicseducation.field_of_studyMaterials sciencePopulationGeneral Physics and AstronomyP680ChromophorePhotobleachingAcceptorCondensed Matter::Materials ScienceFörster resonance energy transferExcited statePhysics::Chemical PhysicsAtomic physicseducationExcitationdescription
We report on the control of the energy transfer pathway in individual donor-acceptor dyads by proper timing of light pulses matching the donor and acceptor transition frequencies, respectively. Excitation of both chromophores at virtually the same time induces efficient singlet-singlet annihilation, whereby excitation energy effectively flows from the acceptor to the donor. The dual pulse excitation scheme implemented here allows for all-optical switching of the fluorescence intensity at the single-molecule level. The population of higher excited states at the donor site was found to significantly increase the photobleaching probability.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2009-06-10 | Physical review letters |