6533b7d2fe1ef96bd125ec55
RESEARCH PRODUCT
Evidence for shifts in the structure and abundance of the microbial community in a long-term PCB-contaminated soil under bioremediation.
Laurent PhilippotDubravka HršakNikolina Udiković-kolićDavid BruFabrice Martin-laurentInes Petrićsubject
BioaugmentationEnvironmental Engineeringpolychlorinated biphenyls ; bioremediation ; microbial community structure ; quantitative PCR ; ribosomal intergenic spacer analysisengineering[SDV]Life Sciences [q-bio]Health Toxicology and MutagenesisRibosomal Intergenic Spacer analysis010501 environmental sciencesBiologyReal-Time Polymerase Chain Reactioncomplex mixtures01 natural sciencesenvironmentalmicroorganisme du solActinobacteriaBiostimulation03 medical and health sciencesBioremediationbioremediationSoil functionscivilEnvironmental ChemistryRhodococcusSoil Pollutantsribosomal intergenic spacer analysisWaste Management and DisposalEnvironmental Restoration and RemediationSoil Microbiology0105 earth and related environmental sciencesrelation sol microorganisme2. Zero hunger0303 health sciencessciences and ecology030306 microbiologyEcology15. Life on landbiology.organism_classificationPollutionSoil contaminationPolychlorinated BiphenylsBiodegradation EnvironmentalMicrobial population biologymicrobial community structuresoil restorationEnvironmental chemistryquantitative PCR[SDE]Environmental Sciencesbacteriadescription
International audience; Although the impact of bioremediation of PCB-contaminated sites on the indigenous microbial community is a key question for soil restoration, it remains poorly understood. Therefore, a small-scale bioremediation assay made of (a) a biostimulation treatment with carvone, soya lecithin and xylose and (b) two bioaugmentation treatments, one with a TSZ7 mixed culture and another with a Rhodococcus sp. Z6 pure strain was set up. Changes in the structure of the global soil microbial community and in the abundances of different taxonomic phyla were monitored using ribosomal intergenic spacer analysis (RISA) and real-time PCR. After an 18-month treatment, the structure of the bacterial community in the bioremediated soils was significantly different from that of the native soil. The shift observed in the bacterial community structure using RISA analysis was in accordance with the monitored changes in the abundances of 11 targeted phyla and classes. Actinobacteria, Bacteriodetes and α- and γ-Proteobacteria were more abundant under all three bioremediation treatments, with Actinobacteria representing the dominant phylum. Altogether, our results indicate that bioremediation of PCB-contaminated soil induces significant changes in the structure and abundance of the total microbial community, which must be addressed to implement bioremediation practices in order to restore soil functions.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-01-01 | Journal of hazardous materials |