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RESEARCH PRODUCT
Interleukin-2 Functionalized Nanocapsules for T Cell-Based Immunotherapy.
Katharina LandfesterStefanie U. FrickVolker MailänderGrit BaierFrederik R. WurmMatthias P. DomogallaKerstin Steinbrinksubject
0301 basic medicineInterleukin 2Materials sciencemedicine.medical_treatmentT cellmedia_common.quotation_subjectGeneral Physics and Astronomy02 engineering and technologyNanocapsules03 medical and health sciencesImmune systemIn vivomedicineGeneral Materials ScienceInternalizationmedia_commonGeneral EngineeringImmunotherapy021001 nanoscience & nanotechnologyCell biology030104 developmental biologyCytokinemedicine.anatomical_structureImmunology0210 nano-technologymedicine.drugdescription
A major demand on immunotherapy is the direct interference with specific immune cells in vivo. In contrast to antibody-engineered nanoparticles to control dendritic cells function, targeting of T cells for biomedical applications still remains an obstacle as they disclose reduced endocytic activities. Here, by coupling the cytokine interleukin-2 (IL-2) to the surface of hydroxyethyl starch nanocapsules, we demonstrated a direct and specifc T cell targeting in vitro and in vivo by IL-2 receptor-mediated internalization. For this purpose, defined amounts of azide-functionalized IL-2 were linked to alkyne-functionalized hydroxyethyl starch nanocapsules via copper-free click reactions. In combination with validated quantification of the surface-linked IL-2 with anthracen azide, this method allowed for engineering IL-2-functionalized nanocapsules for an efficient targeting of human and murine T cell populations with various IL-2 receptor affinities. This nanocapsule-mediated technique is a promising strategy for T cell-based immunotherapies and may be translated to other cytokine-related targeting systems.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-10-11 |