6533b7d2fe1ef96bd125f5c6

RESEARCH PRODUCT

Imaging of Orthotopic Glioblastoma Xenografts in Mice Using a Clinical CT Scanner: Comparison with Micro-CT and Histology

Martin KramerAndreas HugA. ArnsChristoph GrodenManuela C. FelixFrank A. GiordanoStefanie KirschnerGerhard GlattingBettina MürleMarc A. BrockmannFrederik Wenz

subject

MalePathologyCancer Treatmentlcsh:MedicineContrast MediaMice SCIDSignal-To-Noise RatioDiagnostic Radiology030218 nuclear medicine & medical imagingchemistry.chemical_compound0302 clinical medicineMice Inbred NODMedicine and Health Scienceslcsh:ScienceSmall AnimalsTomographyNeurological TumorsMice KnockoutMultidisciplinarymedicine.diagnostic_testBrain NeoplasmsRadiology and ImagingBrainGliomaMagnetic Resonance ImagingIn Vivo ImagingOncologyNeurology030220 oncology & carcinogenesisFemaleAnatomyPreclinical imagingResearch ArticleInterleukin Receptor Common gamma Subunitmedicine.medical_specialtyHistologyImaging TechniquesAnimal TypesTransplantation HeterologousIomeprolBrain tumorNeuroimagingResearch and Analysis Methods03 medical and health sciencesDiagnostic MedicineCell Line TumorGliomamedicineAnimalsHumansMultislicebusiness.industrylcsh:ROrganismsBiology and Life SciencesCancers and NeoplasmsReproducibility of ResultsMagnetic resonance imagingX-Ray Microtomographymedicine.diseaseComputed Axial TomographyIopamidolTransplantationSignal-to-noise ratio (imaging)chemistrylcsh:QGlioblastomabusinessNuclear medicineZoologyNeuroscience

description

Purpose There is an increasing need for small animal in vivo imaging in murine orthotopic glioma models. Because dedicated small animal scanners are not available ubiquitously, the applicability of a clinical CT scanner for visualization and measurement of intracerebrally growing glioma xenografts in living mice was validated. Materials and Methods 2.5x106 U87MG cells were orthotopically implanted in NOD/SCID/ᵞc-/- mice (n = 9). Mice underwent contrast-enhanced (300 μl Iomeprol i.v.) imaging using a micro-CT (80 kV, 75 μAs, 360° rotation, 1,000 projections, scan time 33 s, resolution 40 x 40 x 53 μm) and a clinical CT scanner (4-row multislice detector; 120 kV, 150 mAs, slice thickness 0.5 mm, feed rotation 0.5 mm, resolution 98 x 98 x 500 μm). Mice were sacrificed and the brain was worked up histologically. In all modalities tumor volume was measured by two independent readers. Contrast-to-noise ratio (CNR) and Signal-to-noise ratio (SNR) were measured from reconstructed CT-scans (0.5 mm slice thickness; n = 18). Results Tumor volumes (mean±SD mm3) were similar between both CT-modalities (micro-CT: 19.8±19.0, clinical CT: 19.8±18.8; Wilcoxon signed-rank test p = 0.813). Moreover, between reader analyses for each modality showed excellent agreement as demonstrated by correlation analysis (Spearman-Rho >0.9; p<0.01 for all correlations). Histologically measured tumor volumes (11.0±11.2) were significantly smaller due to shrinkage artifacts (p<0.05). CNR and SNR were 2.1±1.0 and 1.1±0.04 for micro-CT and 23.1±24.0 and 1.9±0.7 for the clinical CTscanner, respectively. Conclusion Clinical CT scanners may reliably be used for in vivo imaging and volumetric analysis of brain tumor growth in mice.

https://doi.org/10.1371/journal.pone.0165994