6533b7d3fe1ef96bd125ffb6
RESEARCH PRODUCT
Deep Venous Thrombosis: Leukocyte Rheology at Baseline and after in vitro Activation
G. CaimiMaria MontanaRosalia LoprestiFilippo FerraraBaldassare Caninosubject
AdultMalePathologymedicine.medical_specialtyMembrane FluidityNeutrophileDeep veinBiologyGranulocytePathogenesisPhysiology (medical)LeukocytesmedicineMembrane fluidityHumansCells CulturedAgedVascular diseaseHematologyMiddle AgedThrombophlebitismedicine.diseaseThrombosisChemotaxis LeukocyteVenous thrombosismedicine.anatomical_structureImmunologyCalciumFemaleRheologydescription
We evaluated leukocyte rheology, expressed as leukocyte filtration, polymorphonuclear (PMN) membrane fluidity and cytosolic Ca<sup>2+</sup> concentration in subjects with acute deep venous leg thrombosis (DVT). In 14 subjects with leg DVT we examined the leukocyte filtration [unfractionated, mononuclear cells (MN), PMNs], PMN membrane fluidity and PMN cytosolic Ca<sup>2+</sup> concentration. Subsequently, we evaluated the same PMN variables after in vitro chemotactic activation with 4-phorbol-12-myristate-13-acetate. At baseline, we observed a significant difference in the filtration of unfractionated and MNs and in PMN cytosolic Ca<sup>2+</sup> concentration. After PMN activation, a significant variation, greater in DVT subjects, was present in PMN filtration at 5 and 15 min. In normals, no variation was present in PMN membrane fluidity or cytosolic Ca<sup>2+</sup> concentration after activation, while in subjects with DVT we found a significant variation in both PMN parameters. These results underline that there is a systemic leukocyte functional alteration in DVT.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2001-01-13 | Pathophysiology of Haemostasis and Thrombosis |