Application of accelerated solvent extraction followed by gel performance chromatography and high-performance liquid chromatography for the determination of polycyclic aromatic hydrocarbons in mussel tissue.

6533b7d3fe1ef96bd125ffb7

RESEARCH PRODUCT

Application of accelerated solvent extraction followed by gel performance chromatography and high-performance liquid chromatography for the determination of polycyclic aromatic hydrocarbons in mussel tissue.

Pedro Martí A. Pastor Olga Pardo Vicent Yusà

subject

Hot Temperature Time Factors Health Toxicology and Mutagenesis Food Contamination Toxicology High-performance liquid chromatography media_common.cataloged_instance Animals European union Organic Chemicals Polycyclic Aromatic Hydrocarbons Chromatography High Pressure Liquid media_common chemistry.chemical_classification Detection limit Chromatography Tissue Extracts Extraction (chemistry)Public Health Environmental and Occupational Health General Chemistry Mussel Contamination Bivalvia Hydrocarbon Freeze Drying chemistry Chemistry (miscellaneous)Chromatography Gel Solvents Environmental Pollutants Saponification Food Science

description

Accelerated solvent extraction (ASE) has been evaluated as a fast alternative to methanolic saponification for the extraction of 12 polycyclic aromatic hydrocarbons (PAHs) from mussel tissue. Several solvent systems and different operating conditions were investigated. The mixture dichloromethane-acetone (1:1, v/v) gave the best recoveries at 125 degrees C and 1500 psi, in a total time of 10 min. No yield difference was found between freeze-drying (Fd) or drying the wet mussel with diatomaceous earth (Ded) prior to extraction. The ASE method was validated using the standard reference material SRM 2977, a freeze-dried mussel tissue with naturally present organic contaminants. The performance characteristics of the ASE method (trueness: 70-110%; precision: 4-14% and limit of quantification (LOQ): 0.1-0.25 microg/kg) meet the criteria established by the European Union for quantitative methods of analysis for official control of organic residues and contaminants. ASE provides a 24 times faster extraction than MSE and reduces 12 times the volume of solvent required.

year	journal	country	edition	language
2005-07-16	Food additives and contaminants

10.1080/02652030500077452 https://pubmed.ncbi.nlm.nih.gov/16019821

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