6533b7d3fe1ef96bd1260102
RESEARCH PRODUCT
H7, a protein kinase C inhibitor, increases the glutathione content of neuroblastoma cells
María Dolores MiñanaFrancisco J. RomeroJuan LlopisVicente FelipoJoaquín RomáSantiago Grisoliasubject
BiophysicsBiologyBiochemistryPiperazinesCellular differentiationchemistry.chemical_compoundMiceNeuroblastomaAlkaloidsStructural BiologySphingosineProtein kinase C1-(5-Isoquinolinesulfonyl)-2-MethylpiperazineGeneticsmedicineTumor Cells CulturedStaurosporineAnimalsNeuroblastoma cellMolecular BiologyProtein kinase CSulfonamidesSphingosineKinaseCell BiologyGlutathioneIsoquinolinesStaurosporineMolecular biologyGlutathioneEnzyme ActivationBiochemistrychemistryEnzyme inhibitor1-(5-Isoquinolinylsulfonyl)-2-methylpiperazine1-(5-Isoquinolinesulfonyl)-2-Methylpiperazinebiology.proteinH7Intracellularmedicine.drugdescription
AbstractIt is shown that the intracellular glutathione (GSH) concentration of neuroblastoma-2a cells in culture increases with a maximum at 24 h after starting treatment with 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7), an inhibitor of protein kinase C (PKC). Other inhibitors of this and other protein kinases, e.g. sphingosine, staurosporine, and HA 1004, at the concentrations tested, had a less marked or negligible effect on intracellular GSH concentration. 12-O-Tetradecanoylphorbol-13-acetate (TPA) was also tested and showed no significant effect 24 h after addition.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-05-25 | FEBS Letters |