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RESEARCH PRODUCT
Immunonephelometric determination of the apolipoprotein A-II.
Winfried PrellwitzC LuleyGerd Hafnersubject
Radial immunodiffusionChromatographyApolipoprotein BbiologyChemistryHuman apolipoproteinBiochemistry (medical)Clinical BiochemistryApolipoprotein A-IIeducationGeneral MedicineSample volumeEvaluation Studies as TopicNephelometry and Turbidimetrybiology.proteinImmunologic TechniquesHumansNephelometryApolipoprotein A-IIApolipoproteins Adescription
A fully mechanized immunonephelometric method is described for the rapid and specific determination of apolipoprotein A-II in serum. The method utilizes commercially available sheep antiserum against human apolipoprotein A-II. Nephelometry was performed with the Behring Nephelometer Analyzer (BNA). A single determination can be performed in 12 minutes, requiring 10 microliters sample volume. The measuring range is about 0.08 to 1.25 g/l apolipoprotein A-II. Precision is characterized by intra-assay coefficients of variation of 3.37%, 3.93% and 4.49% for apolipoprotein A-II concentrations of 1.22 g/l, 0.376 g/l and 0.185 g/l, and inter-assay coefficients of variation of 4.27% for an apolipoprotein A-II concentration of 0.404 g/l, respectively. Accuracy of the method is shown by the close correlation of results with those from radial immunodiffusion (r = 0.913, y = 1.091 x -0.033, n = 75).
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1989-09-01 | Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie |