Bakteriofāga Enc34 replikācijas moduļa proteīnu ekspresija, attīrīšana un raksturošana

6533b7d3fe1ef96bd126045d

RESEARCH PRODUCT

Bakteriofāga Enc34 replikācijas moduļa proteīnu ekspresija, attīrīšana un raksturošana

Elīna ČErnooka

subject

Proteīnu attīrīšana Rentgenstaru kristalogrāfija Bakteriofāgi DNS replikācija Bioloģija Rekombinantu proteīnu ekspresija

description

Darbā tika veikta bakteriofāga Enc34 replikācijas moduļa proteīnu sekvenču analīze un hipotētisko proteīnu ORF3, ORF4, ORF5 un ORF6 eksperimentāla izpēte ar mērķi identificēt to funkcijas. Pētījumu rezultātā praimāzei ORF1 tika atklāts iepriekš neidentificēts helikāzes domēns un ORF8 tika re-anotēts kā resolvāze. Rekombinanti producēts ORF4 bija nešķīstošs, savukārt ORF3 un ORF5 – šķīstoši, un to sekmīga attīrīšana ļauj darbu turpināt ar proteīnu kristalizāciju. ORF6 un delēcijas variantam ORF6Δ21 tika noteiktas kristālstruktūras, kuru analīze kopā ar in vitro eksperimentiem parādīja, ka ORF6 ir ssDNS saistošais proteīns (SSB). Pētījumā pirmoreiz tika atklāta SSB C-terminālā reģiona nozīme ssDNS saistīšanas specifiskuma nodrošināšanai. Darbs tika izstrādāts Latvijas Biomedicīnas pētījumu un studiju centrā Dr. biol. A. Kazāka vadībā.

year	journal	country	edition	language
2016-01-01

https://dspace.lu.lv/dspace/handle/7/33210

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