Differential distribution and enrichment of non-coding RNAs in exosomes from normal and Cancer-associated fibroblasts in colorectal cancer.

6533b7d3fe1ef96bd1260bab

RESEARCH PRODUCT

Differential distribution and enrichment of non-coding RNAs in exosomes from normal and Cancer-associated fibroblasts in colorectal cancer.

Antonio Candia Julie Earl Trinidad Caldés Félix Bonilla María Jesús Larriba Marta Rodríguez Vanesa García-barberán Beatriz Gil Alberto Herrera Mercedes Herrera Pilar Garre Ricardo Alan Verdú Ramos Carlos Llorens Alfredo Carrato Cristina Peña Mercedes Rodríguez-garrote

subject

0301 basic medicine Cancer Research Stromal cell RNA Untranslated Colorectal cancer Biology Exosomes lcsh:RC254-282 Non-coding RNAs 03 medical and health sciences Cancer-Associated Fibroblasts Cell Movement Next generation sequencing medicine Biomarkers Tumor Humans Liquid biopsy Letter to the Editor Cells Cultured Cell Proliferation Tumor microenvironment Colon Cancer Liquid biopsy Sequence Analysis RNA Cancer High-Throughput Nucleotide Sequencing Fibroblasts medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Prognosis Microvesicles Gene Expression Regulation Neoplastic 030104 developmental biology Oncology Tumor microenvironment Tumor progression Cancer research Molecular Medicine Cancer-Associated Fibroblasts Colorectal Neoplasms

description

Exosome production from cancer-associated fibroblasts seems to be an important driver of tumor progression. We report the first in-depth biotype characterization of ncRNAs, analyzed by Next Generation Sequencing and Bioinformatics, expressed in established primary human normal and cancer-associated fibroblasts (CAFs) from cancer and normal mucosa tissues from 9 colorectal cancer patients, and/or packaged in their derived exosomes. Differential representation and enrichment analyses based on these ncRNAs revealed a significant number of differences between the ncRNA content of exosomes and the expression patterns of the normal and cancer-associated fibroblast cells. ncRNA regulatory elements are specifically packaged in CAF-derived exosomes, supporting a specific cross-talk between CAFs and colon cancer cells and/or other stromal cells, mediated by exosomes. These sncRNAs are potential biomarkers present in cancer-associated fibroblast-derived exosomes, which should thereby contribute to developing new non-invasive diagnostic, prognostic and predictive methods for clinical applications in management of cancer patients.

year	journal	country	edition	language
2018-01-01

https://hdl.handle.net/20.500.12530/43138