6533b7d3fe1ef96bd1260be0
RESEARCH PRODUCT
RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues
Maria Antonietta RagusaMaurizio AvernaDavide NotoFrancesca FayerAngelo B. CefalùFabrizio GianguzzaSalvatore TravaliSalvatore Costasubject
In situ hybridizationBiologyß-2-glycoprotein I apoH antiphospholipid syndrome Fanconi syndromeKidneyGeneticsmedicineAnimalsHumansBeta 2-Glycoprotein ITissue DistributionRNA MessengerNorthern blotRats WistarCells CulturedIn Situ HybridizationGlycoproteinsSouthern blotReverse Transcriptase Polymerase Chain ReactionGene Expression ProfilingMyocardiumKidney metabolismGeneral MedicineMolecular biologySmall intestineRatsJejunumReal-time polymerase chain reactionmedicine.anatomical_structureLiverbeta 2-Glycoprotein IApolipoprotein Hdescription
In this study, by using different techniques (i.e. Northern blot hybridization, RT-PCR and Southern blot hybridization) on various normal rat tissues, we were able to identify liver, kidney, heart, small intestine, brain, spleen, stomach and prostate as tissues in which the ApoH gene is transcribed. Moreover, for some of these tissues, by in situ hybridization, we found a specific localization of apoH transcripts. For instance epithelial cells of the bile ducts in liver and of the proximal tubules in kidney are the major sites of apoH synthesis. Our data suggest that some of the different physiological roles proposed for apoH could correlate with its direct expression, while others could correlate with its absorption from bloodstream or adjacent cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-01-01 |