6533b7d3fe1ef96bd12613af
RESEARCH PRODUCT
Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus - (1)H Nuclear Magnetic Resonance search for candidate biomarkers.
Marzenna BartoszewiczGrzegorz ZiółkowskiDanuta SmutnickaStanislaw DejaPatrycja SzymczykPiotr MłynarzAdam Junkasubject
Staphylococcus aureusMagnetic Resonance SpectroscopyMetaboliteBiologymedicine.disease_causeGeneral Biochemistry Genetics and Molecular BiologyMicrobiologychemistry.chemical_compoundMetabolomicsNuclear magnetic resonancemedicineMetabolomeIsoleucineButylene GlycolsRadioisotopesAlanineBiofilmNuclear magnetic resonance spectroscopyStaphylococcal InfectionsBiochemistrychemistryStaphylococcus aureusBiofilmsMetabolomeOsmoprotectantIsoleucineTomography X-Ray ComputedBiomarkersHydrogendescription
Staphylococcus aureus is responsible for many types of infections related to biofilm presence. As the early diagnostics remains the best option for prevention of biofilm infections, the aim of the work presented was to search for differences in metabolite patterns of S. aureus ATCC6538 biofilm vs. free-swimming S. aureus planktonic forms. For this purpose, Nuclear Magnetic Resonance (NMR) spectroscopy was applied. Data obtained were supported by means of Scanning Electron Microscopy, quantitative cultures and X-ray computed microtomography. Metabolic trends accompanying S. aureus biofilm formation were found using Principal Component Analysis (PCA). Levels of isoleucine, alanine and 2,3-butanediol were significantly higher in biofilm than in planktonic forms, whereas level of osmoprotectant glycine-betaine was significantly higher in planktonic forms of S. aureus. Results obtained may find future application in clinical diagnostics of S. aureus biofilm-related infections.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-10-31 | Acta Biochimica Polonica |