Sēnes Lentinula edodes celmu raksturošana pēc lignināžu aktivitātes

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Sēnes Lentinula edodes celmu raksturošana pēc lignināžu aktivitātes

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Lentinula edodes ir plaši kultivējama sēne, kura sintezē enzīmus spējīgus degradēt koksnē esošo lignīnu. Šī pētījuma mērķis ir atlasīt Lentinula edodes celmus pēc to spējas sintezēt ligninolītiskos enzīmus un degradēt lignīnu. Vispirms tika veikts 15 L. edodes celmu skrīnings lignīnu degradējošo enzīmu notektēšanai, tad celmi tika audzēti barotnē ar un bez lignocelulozes (LC) piedevas un noteikta enzīma lakkāzes aktivitāte. Tika noteikts, ka visi L. edodes celmi sintezē divu veidu lignīnu degradējošos enzīmus – lakkāzi un mangāna peroksidāzi. Celmu augšanas intensitāte būtiski neatšķīrās, augot uz agarizētās barotnes ar un bez LC piedevas, bet celmu micēlija biomasa pieauga līdz pat 2 reizēm, augot šķidrajā barotnē ar LC piedevu. Enzīmus ar vilielāko aktivitāti sintezēja celmi 386, 395, 3565 un 4b2c.

year	journal	country	edition	language
2013-01-01

https://dspace.lu.lv/dspace/handle/7/22954

PEPhysical Sciences and Engineering
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