6533b7d4fe1ef96bd1261d7e

RESEARCH PRODUCT

Influence of partial O 2 pressure on the adhesion, proliferation, and osteogenic differentiation of human dental pulp stem cells on β-tricalcium phosphate scaffold

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Our purpose was to analyse the influence of O2 pressure on the adhesion, proliferation, and osteogenic differentiation of human dental pulp stem cells (DPSC) on 3-tricalcium phosphate (3-TCP) scaffold. DPSC isolated from extracted third molars were seeded on 3-TCP and cultured under 3% or 21% O2 pressure. Cell adhesion, proliferation, and differentiation over the biomaterial were evaluated at 7, 13, 18, and 23 days of culture. Cell adhesion was determined by light microscopy, proliferation by DNA quantification, and osteogenic differentiation by alkaline phosphatase activity analysis. All DPSC adhered to 3-TCP with both O2 conditions. Cell proliferation values were higher at 3% O2 in each time point from day 7. ALP activity was not detectable at 7 days. There was, however, an increase in ALP activity over time in both groups. At 13, 18, and 23 days of culture, higher ALP activity was recorded under 3% O2 pressure. DPSC display capacity of adhering to 3-tricalcium phosphate under 3% or 21% O2 pressure conditions. Cell proliferation on 3-tricalcium phosphate was significantly reduced under oxidative stress conditions, which is 21% O2, the most frequently used O2 tension. 3-TCP can itself promote osteogenic differentiation of DPSC and is enhanced under 3% compared with 21% O2.