The phosphorylated pathway of serine biosynthesis is essential both for male gametophyte and embryo development and for root growth in Arabidopsis.

6533b7d4fe1ef96bd1261dd2

RESEARCH PRODUCT

The phosphorylated pathway of serine biosynthesis is essential both for male gametophyte and embryo development and for root growth in Arabidopsis.

Armand D. Anoman Alisdair R. Fernie Borja Cascales-miñana Sonia Osorio María Flores-tornero José Pertusa Manuel Alaiz Roc Ros Jesús Muñoz-bertomeu Juan Segura

subject

Mutant Citric Acid Cycle Green Fluorescent Proteins Immunoblotting Arabidopsis Plant Science Biology Plant Roots Serine Microspore Microscopy Electron Transmission Gene Expression Regulation Plant Arabidopsis Serine Arabidopsis thaliana Amino Acids Phosphorylation Research Articles Tapetum Arabidopsis Proteins Reverse Transcriptase Polymerase Chain Reaction Gene Expression Regulation Developmental Embryo Phosphoserine phosphatase Cell Biology biology.organism_classification Plants Genetically Modified Phosphoric Monoester Hydrolases Biosynthetic Pathways Biochemistry Microscopy Fluorescence Mutation Seeds Pollen Glycolysis

description

This study characterizes the phosphorylated pathway of Ser biosynthesis (PPSB) in Arabidopsis thaliana by targeting phosphoserine phosphatase (PSP1), the last enzyme of the pathway. Lack of PSP1 activity delayed embryo development, leading to aborted embryos that could be classified as early curled cotyledons. The embryo-lethal phenotype of psp1 mutants could be complemented with PSP1 cDNA under the control of Pro35S (Pro35S:PSP1). However, this construct, which was poorly expressed in the anther tapetum, did not complement mutant fertility. Microspore development in psp1.1/psp1.1 Pro35S:PSP1 arrested at the polarized stage. The tapetum from these lines displayed delayed and irregular development. The expression of PSP1 in the tapetum at critical stages of microspore development suggests that PSP1 activity in this cell layer is essential in pollen development. In addition to embryo death and male sterility, conditional psp1 mutants displayed a short-root phenotype, which was reverted in the presence of Ser. A metabolomic study demonstrated that the PPSB plays a crucial role in plant metabolism by affecting glycolysis, the tricarboxylic acid cycle, and the biosynthesis of amino acids. We provide evidence of the crucial role of the PPSB in embryo, pollen, and root development and suggest that this pathway is an important link connecting primary metabolism with development.

year	journal	country	edition	language
2013-06-01	The Plant cell

10.1105/tpc.113.112359 https://pubmed.ncbi.nlm.nih.gov/23771893