0000000000222747

AUTHOR

Manuel Alaiz

showing 2 related works from this author

The phosphorylated pathway of serine biosynthesis is essential both for male gametophyte and embryo development and for root growth in Arabidopsis.

2013

This study characterizes the phosphorylated pathway of Ser biosynthesis (PPSB) in Arabidopsis thaliana by targeting phosphoserine phosphatase (PSP1), the last enzyme of the pathway. Lack of PSP1 activity delayed embryo development, leading to aborted embryos that could be classified as early curled cotyledons. The embryo-lethal phenotype of psp1 mutants could be complemented with PSP1 cDNA under the control of Pro35S (Pro35S:PSP1). However, this construct, which was poorly expressed in the anther tapetum, did not complement mutant fertility. Microspore development in psp1.1/psp1.1 Pro35S:PSP1 arrested at the polarized stage. The tapetum from these lines displayed delayed and irregular devel…

MutantCitric Acid CycleGreen Fluorescent ProteinsImmunoblottingArabidopsisPlant ScienceBiologyPlant RootsSerineMicrosporeMicroscopy Electron TransmissionGene Expression Regulation PlantArabidopsisSerineArabidopsis thalianaAmino AcidsPhosphorylationResearch ArticlesTapetumArabidopsis ProteinsReverse Transcriptase Polymerase Chain ReactionGene Expression Regulation DevelopmentalEmbryoPhosphoserine phosphataseCell Biologybiology.organism_classificationPlants Genetically ModifiedPhosphoric Monoester HydrolasesBiosynthetic PathwaysBiochemistryMicroscopy FluorescenceMutationSeedsPollenGlycolysisThe Plant cell
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An interlaboratory study as useful tool for proficiency testing of chemical oxygen demand measurements using solid substrates and liquid samples with…

2009

In 2008, the first Proficiency Testing Scheme of Chemical Oxygen Demand (1stCOD-PTADG) was conducted to assess the results obtained for different research groups whose field work is mainly anaerobic digestion. This study was performed using four samples, two solid samples as raw materials and two solid samples to prepare high concentration suspended solid solutions. Invitations were sent to a large number of laboratories, mainly to anaerobic digestion research groups. Finally, thirty labs from sixteen countries agreed to participate, but for different reasons four participants could not send any data. In total, twenty-six results were reported to the COD-PT coordinator. This study showed th…

Sample (material)[SDV]Life Sciences [q-bio]Analytical chemistryCarbohydratesSolid substrateSuspended solidsHomogeneity test010501 environmental sciences01 natural sciencesStandard deviationChemistry Techniques AnalyticalAnalytical ChemistryProficiency testingFatsAnaerobic digestionStatisticsProficiency testingPlant OilsSunflower OilAnaerobiosisChenopodium quinoa0105 earth and related environmental sciencesSuspended solidsInterlaboratory studyScience & TechnologySewageChemistryClinical Laboratory Techniques010401 analytical chemistryChemical oxygen demandProteinsReproducibility of ResultsCHEMICAL OXYDEN DEMANDReference StandardsTheoretical oxygen demand0104 chemical sciencesOxygenCHIMIE ANALYTIQUECertified reference materials[SDE]Environmental SciencesChemical oxygen demandRound robin test
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