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RESEARCH PRODUCT
Factors affecting the production of putrescine from agmatine by Lactobacillus hilgardii X1B isolated from wine
Sergi FerrerI. PardoMario Eduardo ArenaMario Eduardo ArenaJosé María LandeteM. C. Manca De NadraM. C. Manca De Nadrasubject
ved/biology.organism_classification_rank.speciesLactobacillus hilgardiiApplied Microbiology and BiotechnologyCiencias Biológicas//purl.org/becyt/ford/1 [https]chemistry.chemical_compoundBiología Celular MicrobiologíaLACTIC ACID BACTERIABiogenic amine//purl.org/becyt/ford/1.6 [https]chemistry.chemical_classificationWineved/biologyfood and beveragesBIOGENIC AMINESWINEGeneral MedicinePUTRESCINEAmino acidLactic acidAgmatine deiminasechemistryBiochemistryPutrescineAGMATINE DEIMINASEAgmatineCIENCIAS NATURALES Y EXACTASBiotechnologydescription
Aims: To elucidate and characterize the metabolic putrescine synthesis pathway from agmatine by Lactobacillus hilgardii X1B. Methods and Results: The putrescine formation from agmatine by resting cells (the normal physiological state in wine) of lactic acid bacteria isolated from wine has been determined for the first time. Agmatine deiminase and N-carbamoylputrescine hydrolase enzymes, determined by HPLC and LC-Ion Trap Mass Spectrometry, carried out the putrescine synthesis from agmatine. The influence of pH, temperature, organic acids, amino acids, sugars and ethanol on the putrescine formation in wine was determined. Conclusions: Resting cells of Lact. hilgardii X 1B produce putrescine in wine. The putrescine production was carried out from agmatine through the agmatine deiminase system. Significance and Impact of the Study: These results have significance from two points of view, wine quality and toxicological and microbiological aspects, taking account that putrescine, which origin is still controversial, is quantitatively the main biogenic amine found in wine. © 2008 The Authors. Fil: Arena, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina Fil: Landete, J. M.. Universidad de Valencia; España Fil: Manca, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Pardo, I.. Universidad de Valencia; España Fil: Ferrer, S.. Universidad de Valencia; España
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-07-01 |