6533b7d4fe1ef96bd1261f90

RESEARCH PRODUCT

Heterogeneity within and between primary colorectal carcinomas and matched metastases as revealed by analysis of Ki-ras and p53 mutations

Antonio RussoManuela MigliavaccaPaolo ColombaIda AlbaneseViviana BazanRosa Maria TomasinoMario La FarinaMarcello TagliaviaAngelo G. Scibetta

subject

p53Colorectal cancerDNA Mutational AnalysisStatistics as TopicBiophysicsKi-raBiologyOncogene Protein p21(ras)medicine.disease_causeBiochemistryMetastasisMetastasischemistry.chemical_compoundSequence Homology Nucleic AcidmedicineHumansMolecular BiologyGeneRegulation of gene expressionMutationGene Expression ProfilingCarcinomaLiver NeoplasmsCell BiologySequence Analysis DNAmedicine.diseasePrimary tumorGene expression profilingGene Expression Regulation NeoplasticColorectal carcinomaGenes raschemistryMutationCancer researchTumor Suppressor Protein p53Colorectal NeoplasmsDNA

description

Analysis of the genetic status of Ki-ras and p53 in primary colorectal carcinomas and matched colorectal liver metastasis from 30 patients reveals an overall heterogeneity both within and between the two tumoral tissues. Both genes were found mutated with a similar frequency in both tissues; however, identical mutations in primary tumor and matched metastasis were found less frequently in the case of the Ki-ras than the p53 gene. Only in three cases the same p53 and Ki-ras mutations found in the primary tumor were found also in the metastasis. In several metastatic specimens the DNA bearing a mutation detected also in the primary tumor appears significantly less abundant than the wild-type DNA. These data are discussed in the light of current models of primary tumor/metastasis relationships.

yearjournalcountryeditionlanguage
2004-10-16
10.1016/j.bbrc.2004.10.111http://hdl.handle.net/10447/4697