6533b7d4fe1ef96bd12629e3
RESEARCH PRODUCT
HSV hepatitis in the mouse: A light and electron microscopic study with immunohistology and in situ hybridization
P. SchirmacherHans-peter DienesD. FalkeW. ThoenesK. LübbeM. Wörsdörfersubject
Pathologymedicine.medical_specialtyvirusesIn situ hybridizationBiologyVirus Replicationmedicine.disease_causeVirusLeukocyte CountMiceAlphaherpesvirinaemedicineAnimalsLymphocytesCytopathic effectHepatitisMice Inbred BALB CNucleic Acid HybridizationHerpes SimplexHistologyDNAGeneral Medicinemedicine.diseasebiology.organism_classificationImmunohistochemistryMicroscopy ElectronHerpes simplex virusLiverHepatitis Viral AnimalFemaleViral hepatitisdescription
In order to characterize better the morphology and immune response in acute necrotizing HSV infection, murine HSV hepatitis was examined. BALB/c mice were inoculated intraperitoneally with 10(6) plaque-forming units (PFU) of HSV-1 (Lenette) and HSV-2 (D316). In both groups half the animals were pretreated with silica particles to block macrophage function. Up to 6 days after infection four mice from each group were sacrificed at daily intervals and the livers were examined by light and electron microscopy, immunohistology, in situ hybridization, combined immunohistology/in situ hybridization and titration of viral PFU. HSV-2 infected mice developed severe necrotizing hepatitis with persistence of HSV in the liver tissue until the end of the study. HSV-1 infected mice rapidly eliminated the virus and revealed only small necrotic foci. Early phase alterations and necrotic phase lesions were distinguished and characterized and morphologic evidence of a direct cytopathic effect of HSV was detected. A specific immune reaction in late stages appeared to be mediated by T4-positive T-lymphocytes. In situ hybridization and immunohistochemistry showed a close correlation with virus titration and were valuable in characterizing early phases and in the assessment of prognosis and differential diagnosis.
year | journal | country | edition | language |
---|---|---|---|---|
1988-12-01 | Virchows Archiv B Cell Pathology Including Molecular Pathology |