6533b7d4fe1ef96bd1262a1f
RESEARCH PRODUCT
Production of an egg yolk antibody against Parietaria judaica 2 allergen
Giovanni DuroAlessia GalloG. De LeoSimona FontanaMichel BecchiSimona TavernaSimona PrincipeMarilisa BarrancaRiccardo AlessandroGiovanni Cassatasubject
food.ingredientImmunoglobulinsmedicine.disease_causeAntibodieslaw.inventionAllergenfoodlawYolkegg yolk antibodymedicineAnimalsGel electrophoresisDose-Response Relationship DrugbiologyGeneral MedicineAntigens Plantbiology.organism_classificationEgg YolkFusion proteinParietaria judaicaParietariaBiochemistryPolyclonal antibodiesImmunologyRecombinant DNAbiology.proteinParietaria judaicaPollenFemaleAnimal Science and ZoologyAntibodyChickensallergendescription
Specific antibodies are essential tools for studying proteins as well as for diagnostic research in biomedicine. The egg yolk of immunized chicken is an inexpensive source of high-quality polyclonal antibodies. The 12-kDa Parietaria judaica 2 allergen was expressed as a fusion protein and was used to immunize Leghorn chickens. In this paper, we show, using 2-dimensional gel electrophoresis and immunoblotting, that chicken antibodies raised against a recombinant allergen can be used to recognize similar proteins from a pollen raw extract. Allergen identity was confirmed by nanoLC-nanospray-tandem mass spectrometry analysis. Our data demonstrate for the first time that a synergistic combination of molecular biology, 2-dimensional PAGE, and use of nonmammalian antibodies represents a powerful tool for reliable identification of allergens.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2009-07-11 | Poultry Science |