6533b7d4fe1ef96bd12630ed

RESEARCH PRODUCT

Die Konzentration von Noradrenalin und Adrenalin in den einzelnen Abschnitten des Herzens

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description

1. A method for the assay of noradrenaline and adrenaline in small pieces of heart tissue is described. The amines were extracted with acid ethanol and separated by paper chromatography. The regions containing the amines were eluted and the amount of amines in the eluates was determined by bioassay. Noradrenaline was assayed on the blood pressure of the pithed rat. Adrenaline was assayed on the isolated atropinized rat's uterus stimulated with oxytocin. 2. The identity of the substances tested by bioassay with noradrenaline and adrenaline was confirmed by a number of chemical and pharmacological tests. 3. The concentration of catechol amines in different parts of the heart was studied in cats, rabbits, guinea pigs, and rats. In all species investigated, the noradrenaline concentration was much higher in the right atrium than in the left atrium and higher in the right ventricle than in the left ventricle. Approximately 4% of the total amines consisted of adrenaline. 4. The small percentage of adrenaline indicates that the heart of these species contains only little chromaffine tissue. The high concentration of noradrenaline in the right atrium is thought to be mainly due to the presence of adrenergic fibres.