6533b7d4fe1ef96bd126321a
RESEARCH PRODUCT
Inhibition of the system luminol-H2O2-Fe(CN)63− chemiluminescence by the Mn(II) indirect determination of isoniazid in a pharmaceutical formulation
A. Gregorio AlapontL. Lahuerta ZamoraJ. Martínez CalatayudE. Aurechia Giménezsubject
ChromatographyPotassiumBiophysicschemistry.chemical_elementManganesePharmaceutical formulationRedoxlaw.inventionLuminolchemistry.chemical_compoundchemistryPolymerizationChemistry (miscellaneous)lawHydrogen peroxideChemiluminescenceNuclear chemistrydescription
A flow injection procedure for the indirect chemiluminescent determination of isoniazid is proposed. The method is performed in a flow-injection manifold provided with a solid-phase reactor. The reactor was made from manganese dioxide physically entrapped by polymerization; the redox reaction isoniazid-manganese dioxide released Mn(II) which was monitored through its inhibitory effect on the reaction between luminol and hydrogen peroxide in presence of potassium hexacyanoferrate (III). The procedure resulted in a linear calibration graph over the range 5-15 mg/L of isoniazid with a sample throughput of 43 samples/h. The influence of foreign compounds was studied and the method was applied to determination of the drug in a pharmaceutical formulation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-05-01 | Journal of Bioluminescence and Chemiluminescence |