6533b7d4fe1ef96bd126322f
RESEARCH PRODUCT
Scanning near-field optical microscopy using semiconductor nanocrystals as a local fluorescence and fluorescence resonance energy transfer source
George T. ShubeitaGiovanni DietlerInga PotapovaS. K. SekatskiiAlf MewsTh. Baschésubject
HistologyMaterials sciencebusiness.industryNear-field opticsFluorescence correlation spectroscopyFluorescencePathology and Forensic Medicinelaw.inventionFörster resonance energy transferOpticsResonance fluorescenceOptical microscopelawNear-field scanning optical microscopeFluorescence cross-correlation spectroscopybusinessdescription
Local fluorescence probes based on CdSe semiconductor nanocrystals were prepared and tested by recording scanning near-field optical microscopy (SNOM) images of calibration samples and fluorescence resonance energy transfer SNOM (FRET SNOM) images of acceptor dye molecules inhomogeneously deposited onto a glass substrate. Thousands of nanocrystals contribute to the signal when this probe is used as a local fluorescence source while only tens of those (the most apical) are involved in imaging for the FRET SNOM operation mode. The dip-coating method used to make the probe enables diminishing the number of active fluorescent nanocrystals easily. Prospects to realize FRET SNOM based on a single fluorescence centre using such an approach are briefly described.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-06-01 |