6533b7d4fe1ef96bd1263287
RESEARCH PRODUCT
Gonadotropin-releasing hormone (GnRH)-I regulation of interleukin (IL)-1b and IL-1 receptor antagonist expression in cultured human endometrial stromal cells
Francisco RagaEva M. CasañFernando Bonilla-musolessubject
Adultendocrine systemmedicine.medical_specialtyStromal cellmedicine.drug_classmedicine.medical_treatmentInterleukin-1betaGonadotropin-releasing hormoneBiologyEndometriumGonadotropin-Releasing HormoneEndometriumInternal medicinemedicineHumansEmbryo ImplantationRNA MessengerBlastocystProtein PrecursorsReceptorObstetrics and GynecologyInterleukinReceptor antagonistInterleukin 1 Receptor Antagonist ProteinEndocrinologymedicine.anatomical_structureCytokineFemaleStromal Cellshormones hormone substitutes and hormone antagonistsdescription
Aim: Human endometrium is an active site of cytokine production and action. Among these cytokines, the interleukin-1 (IL-1) system seems to be relevant to the embryonic implantation process. We have previously reported the production of GnRH-I by human blastocyst, as well as the presence of GnRH-I receptor in human endometrium. This suggests a close interaction between the immune and endocrine systems through these cytokine mediators in embryonic implantation. Methods: To test the relevance of this interaction during embryonic implantation, we investigated GnRH-I regulation of IL-1b and IL-1ra mRNA and protein expression in human endometrial stromal cells using quantitative competitive polymerase chain reaction and ELISA. Results: IL-1b mRNA and protein expression in cultured human endometrial stromal cells was significantly enhanced by GnRH-agonist in comparison to control groups. IL-1ra mRNA and protein was significantly decreased by GnRH-agonist in comparison to control groups. In contrast, the GnRH-antagonist ablated the regulatory effects of GnRH agonist in 1b and IL-1ra mRNA and protein levels in a dose-dependent manner. Conclusions: In conclusion, these results suggest a possible close interaction between the immune and endocrine systems in human embryonic implantation through the classical neuropeptide hormone GnRH and its receptor.
year | journal | country | edition | language |
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2008-10-22 | Journal of Obstetrics and Gynaecology Research |