6533b7d4fe1ef96bd1263287

RESEARCH PRODUCT

Gonadotropin-releasing hormone (GnRH)-I regulation of interleukin (IL)-1b and IL-1 receptor antagonist expression in cultured human endometrial stromal cells

Francisco RagaEva M. CasañFernando Bonilla-musoles

subject

Adultendocrine systemmedicine.medical_specialtyStromal cellmedicine.drug_classmedicine.medical_treatmentInterleukin-1betaGonadotropin-releasing hormoneBiologyEndometriumGonadotropin-Releasing HormoneEndometriumInternal medicinemedicineHumansEmbryo ImplantationRNA MessengerBlastocystProtein PrecursorsReceptorObstetrics and GynecologyInterleukinReceptor antagonistInterleukin 1 Receptor Antagonist ProteinEndocrinologymedicine.anatomical_structureCytokineFemaleStromal Cellshormones hormone substitutes and hormone antagonists

description

Aim:  Human endometrium is an active site of cytokine production and action. Among these cytokines, the interleukin-1 (IL-1) system seems to be relevant to the embryonic implantation process. We have previously reported the production of GnRH-I by human blastocyst, as well as the presence of GnRH-I receptor in human endometrium. This suggests a close interaction between the immune and endocrine systems through these cytokine mediators in embryonic implantation. Methods:  To test the relevance of this interaction during embryonic implantation, we investigated GnRH-I regulation of IL-1b and IL-1ra mRNA and protein expression in human endometrial stromal cells using quantitative competitive polymerase chain reaction and ELISA. Results:  IL-1b mRNA and protein expression in cultured human endometrial stromal cells was significantly enhanced by GnRH-agonist in comparison to control groups. IL-1ra mRNA and protein was significantly decreased by GnRH-agonist in comparison to control groups. In contrast, the GnRH-antagonist ablated the regulatory effects of GnRH agonist in 1b and IL-1ra mRNA and protein levels in a dose-dependent manner. Conclusions:  In conclusion, these results suggest a possible close interaction between the immune and endocrine systems in human embryonic implantation through the classical neuropeptide hormone GnRH and its receptor.

https://doi.org/10.1111/j.1447-0756.2008.00737.x