6533b7d4fe1ef96bd1263434
RESEARCH PRODUCT
Inhibition of expression of natural UAG suppressor glutamine tRNA in HIV-infected human H9 cells in vitro by Avarol.
Georg HessSusumu NishimuraPrem S. SarinKarl-h. Meyer Zum BüschenfeldePetra ReuterHeinz C. SchröderYoshiyuki KuchinoWerner E.g. Müllersubject
ReticulocytesvirusesGlutamineImmunologyBiologyAntiviral AgentsViruslaw.inventionCell LineSuppression GeneticlawVirologyRNA Transfer GlnGene expressionAnimalsHumansCodonvirus diseasesHIVNucleic Acid HybridizationBiological activitybiochemical phenomena metabolism and nutritionRNA Transfer Amino Acid-SpecificCell Transformation ViralMolecular biologyIn vitroGlutamineTobacco Mosaic VirusInfectious DiseasesCell cultureProtein BiosynthesisTransfer RNASuppressorRNA ViralRabbitsSesquiterpenesdescription
HTLV-IIIB-infected H9 cells are shown to contain a high level of the natural UAG suppressor glutamine tRNA(UmUG Gln); this tRNA has been demonstrated to be required for the synthesis of Moloney murine leukemia virus (Mo-MuLV)-encoded protease. After cultivation of HTLV-IIIB-infected H9 cells with Avarol at a concentration (1 microgram/ml), previously found to protect the cells against the cytopathic effects of HTLV-III, an almost complete inhibition of the synthesis of the tRNA(UmUG Gln) was observed. Moreover, we obtained some evidence that the processing of the HTLV-III precursor protein p53 to p24 is inhibited by Avarol in infected cells, suggesting that the compound interferes with the expression of the viral protease gene.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1988-08-01 | AIDS research and human retroviruses |