6533b7d4fe1ef96bd126349c

RESEARCH PRODUCT

MOESM8 of Reanalysis of Chinese Treponema pallidum samples: all Chinese samples cluster with SS14-like group of syphilis-causing treponemes

subject

description

Additional file 8. The phylogenetic trees of the tp0136 and tp0548 genes. The phylogenetic trees were constructed using the Maximum Likelihood method based on the Tamura–Nei model. The bar scale represents the number of substitutions per site. The analysis involved 14 TPA nucleotide sequences including eight derived from the Chinese samples: SHC-0, SHD-R, SHE-V, SHG-I2, B3, C3, K3, and Q3. The T. pallidum subsp. pertenue Fribourg-Blanc sequence [1] was used as an outgroup. There were totals of 1547 and 1317 positions in the final dataset for tp0136 and tp0548 genes, respectively. For both genes, two separate clusters were identified: one cluster of Nichols-like TPA strains (TPA Lineage 1), and a second cluster of SS14-like TPA strains including all tested Chinese strains (TPA Lineage 3). Both clusters were supported by bootstrap values greater than 95%.