6533b7d5fe1ef96bd1263cdf

RESEARCH PRODUCT

A fully synthetic glycopeptide antitumor vaccine based on multiple antigen presentation on a hyperbranched polymer.

Bastian GerlitzkiMarkus GlaffigHolger FreyHorst KunzEdgar SchmittBjörn PalitzschChristoph SchüllSebastian HartmannLutz Nuhn

subject

GlycerolSynthetic vaccinePolymersAntigen presentationEpitopes T-LymphocyteBreast NeoplasmsCancer VaccinesCatalysisEpitopeAntibodiesMiceImmune systemAntigenAntigens NeoplasmTetanus ToxoidOrganic chemistryAnimalsHumansAntigen PresentationbiologyChemistryOrganic ChemistryMucin-1ToxoidGlycopeptidesGeneral ChemistryGlycopeptideImmunologybiology.proteinMCF-7 CellsClick ChemistryFemaleAntibody

description

For antitumor vaccines both the selected tumor-associated antigen, as well as the mode of its presentation, affect the immune response. According to the principle of multiple antigen presentation, a tumor-associated MUC1 glycopeptide combined with the immunostimulating T-cell epitope P2 from tetanus toxoid was coupled to a multi-functionalized hyperbranched polyglycerol by "click chemistry". This globular polymeric carrier has a flexible dendrimer-like structure, which allows optimal antigen presentation to the immune system. The resulting fully synthetic vaccine induced strong immune responses in mice and IgG antibodies recognizing human breast-cancer cells.

yearjournalcountryeditionlanguage
2014-03-12Chemistry (Weinheim an der Bergstrasse, Germany)
10.1002/chem.201400256https://pubmed.ncbi.nlm.nih.gov/24623572