6533b7d5fe1ef96bd1263e5f

RESEARCH PRODUCT

Development of a modified DNA extraction method for pulsed-field gel electrophoresis analysis of Staphylococcus aureus and enterococci without using lysostaphin

Maryam OmraniBizhan NomanpourMohammad Mehdi FeizabadiArash GhodousiFereshteh Shahcheraghi

subject

Microbiology (medical)DNA BacterialStaphylococcus aureusSettore MED/07 - Microbiologia E Microbiologia ClinicaMicrococcaceaemedicine.disease_causeMicrobiologyMicrobiologyPulsed-field gel electrophoresismedicineHumansMolecular BiologyGel electrophoresisBacteriological TechniquesbiologyLysostaphinbiochemical phenomena metabolism and nutritionSpheroplastStreptococcaceaebiology.organism_classificationBacterial Typing TechniquesElectrophoresis Gel Pulsed-FieldEnterococcusStaphylococcus aureusLysostaphinEnterococcusPulse-field gel electrophoresis(PFGE) MRSA VRE Nosocomial infections

description

A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Staphylococcus aureus and enterococci to reduce the cost of using lysostaphin. This protocol reduces the expenses of PFGE typing of S. aureus and enterococci as it removes the use of lysostaphin during the spheroplast formation from these bacteria.

yearjournalcountryeditionlanguage
2010-07-23
http://hdl.handle.net/10447/77582