6533b7d5fe1ef96bd12646cd

RESEARCH PRODUCT

Biocatalyzed acidolysis of soybean oil triacylglycerols to increase oleic acid content

Lina CossignaniJordi MañesPietro DamianiM. Stella Simonetti

subject

food.ingredientGlycerideTriacylglycerol lipaseSoybean triacylglycerolsRhizomucor mieheiSoybean triacylglycerols; Lipase catalyzed acidolysisBiochemistryCatalysisSoybean oilAnalytical Chemistrychemistry.chemical_compoundfoodLipaseTriglycerideschemistry.chemical_classificationChromatographybiologyOrganic ChemistryFatty acidGeneral Medicinebiology.organism_classificationLipase catalyzed acidolysisSoybean OilOleic acidVegetable oilchemistrybiology.proteinlipids (amino acids peptides and proteins)AcidsOleic Acid

description

Lipase catalyzed acidolysis of triacylglycerols (TAG) of soybean oil with oleic acid in organic solvent was studied; immobilized lipase from Rhizomucor miehei was used and the effects of reaction time, incubation temperature and enzyme load on TAG total and positional fatty acid (FA) percentage compositions were investigated. The results show that oleic acid incorporation was high after 24 and 48 h, while after 72 h a lower level of oleic acid in TAG was observed. Moreover, for the reactions carried out at 30 and 40 degrees C, it was observed that the oleic acid level was about 46.5% while ligtly higher values (about 49%) were observed at 50 degrees C; however, under this last condition, the modification of sn-2 position FA composition was higher. Finally, the variable enzyme load resulted also important on the incorporation of oleic acid; in particular, even if the value of 10% (w/w) of enzyme load resulted in a lightly lower incorporation of oleic acid in soybean oil TAG (about 45%), in this situation a minimal modification of sn-2 position FA composition was obtained.

yearjournalcountryeditionlanguage
2004-11-06Journal of Chromatography A
https://doi.org/10.1016/j.chroma.2004.08.015