6533b7d5fe1ef96bd126490f
RESEARCH PRODUCT
Determination of major carotenoids in vegetables by capillary electrochromatography.
Peter J. SchoenmakersJosé Manuel Herrero-martínezGuillermo Ramis-ramosWim Th. KokSebastiaan Eeltinksubject
chemistry.chemical_classificationLuteinCapillary electrochromatographyChromatographyResolution (mass spectrometry)Molecular StructureElutionReproducibility of ResultsFiltration and SeparationStereoisomerismCarotenoidsLycopeneAnalytical Chemistrychemistry.chemical_compoundElectrochromatographychemistryXanthophyllVegetablesCarotenoidChromatography Micellar Electrokinetic Capillarydescription
A simple and rapid method for the isocratic separation and determination of carotenoids (carotenes and xanthophylls) in vegetables by CEC is described. The capillary column (100 microm ID, 25 cm effective length) was packed with 3 microm Hypersil ODS particles. The optimized mobile phase contained 60% ACN, 35% THF and 5% of a 5 mM Tris aqueous buffer of pH 8. beta-Carotene, lycopene and lutein were separated with efficiencies of 66 000-128 000 plates/m within a short time (less than 12 min for the last peak eluted, 13/13'-cis-beta-carotene). An excellent resolution of the three carotenoids, as well as partial resolution of their geometrical isomers, was achieved. Application to the determination of the analytes in carrot, tomato, spinach and corn was demonstrated.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-01-01 | Journal of separation science |