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RESEARCH PRODUCT

AB1035 INTESTINAL MICROBIOTA COMPOSITION OF ADULT PATIENTS WITH FAMILIAL MEDITERRANEAN FEVER AND HEALTHY CONTROLS (THE RHEUMA-BIOTA STUDY)

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Background:Although Familial Mediterranean Fever (FMF) is a monogenic disease, microbiota composition may play role in the pathogenesis or phenotypic expression.Objectives:We aim to evaluate the intestinal microbiota composition in patients with FMF and to compare with healthy controls.Methods:In this prospective cohort study, a group of 10 adult patients with FMF and 10 age-appropriate healthy controls, for which there was strict inclusion/exclusion, were enrolled. Fecal samples were stored at -80°C until DNA extraction. A region of the 16S rRNA gene (V3-V4) was selected and sequencing was performed on the Illumina MiSeq platform at the Sequencing and Bioinformatics Service of FISABIO foundation.Results:Alpha and beta diversity tests were similar between FMF and control groups except that Chao1 index. Chao1 index was modestly decreased in FMF group comparing the healthy controls (p<0.05). Our results showed differences in the intestinal microbiota composition of patients with FMF, with a higher abundance ofEggerthella, at genus level. At species level,Eggerthella sinensisandEggerthella lentawere more abundant in patients with FMF.Conclusion:Eggerthella lentawas previously shown to be higher in type II diabetes, multiple sclerosis, rheumatoid arthritis and some disseminated infections. In this study we firstly showed abundance ofEggerthellain patients with FMF, especially inE. sinensisandE. lenta;in addition to. Whether any of observed associations are causal, or the direction of causality is unclear yet and further studies with patients with FMF at the first diagnosis might clarify this issue.Disclosure of Interests:None declared