6533b7d5fe1ef96bd1265042

RESEARCH PRODUCT

Effect of Monooxygenase Inducers on the Binding of Benzo-(A)Pyrene Metabolites to Cellular Macromolecules in Perfused Rat Lungs

G. F. KahlE. KlausB. Deckers-schmelzle

subject

chemistry.chemical_compoundBenzo(a)pyrenechemistryBiochemistryRNAPyreneInducerSpecific activityMonooxygenaseDNAMacromolecule

description

The irreversible binding of metabolically activated [3H]-benzo(a)pyrene (BP) to cellular macromolecules of isolated perfused rat lungs was studied. Lungs from differently pretreated animals were perfused in situ in a recirculating system without ventilation. BP with a specific activity of 10 mCi/μmol was added to 50 ml perfusion medium containing 40% washed bovine erythrocytes to a final concentration of 1 μM. DNA, RNA and protein fractions were isolated and assayed for irreversibly bound radioactivity.

yearjournalcountryeditionlanguage
1978-01-01
https://doi.org/10.1007/978-3-662-39532-5_92