6533b7d5fe1ef96bd1265149
RESEARCH PRODUCT
A Robust Generic Method for Grid Detection in White Light Microscopy Malassez Blade Images in the Context of Cell Counting
Ambroise MarinEmmanuel DenimalPaul MolinLudovic JournauxStéphane Guyotsubject
Blade (geometry)Computer scienceMalassezImage processingContext (language use)Saccharomyces cerevisiaeHough transformlaw.inventionSet (abstract data type)symbols.namesakeOpticslawimage analysisMicroscopy[ INFO.INFO-TI ] Computer Science [cs]/Image ProcessingImage Processing Computer-AssistedComputer visionInstrumentationcell segmentationMicroscopybusiness.industrymorphological mathematicsGridFourier transformHough transform[INFO.INFO-TI]Computer Science [cs]/Image Processing [eess.IV]symbolsFourier transformArtificial intelligencebusinessAlgorithmsdescription
AbstractIn biology, cell counting is a primary measurement and it is usually performed manually using hemocytometers such as Malassez blades. This work is tedious and can be automated using image processing. An algorithm based on Fourier transform filtering and the Hough transform was developed for Malassez blade grid extraction. This facilitates cell segmentation and counting within the grid. For the present work, a set of 137 images with high variability was processed. Grids were accurately detected in 98% of these images.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-02-01 |