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RESEARCH PRODUCT

Quantitative Topography of the Hemodynamic-Oxidative Metabolic Interrelation by Multispot Measurement Approach in Rat Ischemia Models

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We applied the multispot measurement approach for both laser Doppler (LD) and a microspectrophotometric method to measure local cerebral blood flow (1CBF) and local tissue hemoglobin oxygenation (IHbO2). We demonstrated that the current technology permits in vivo observation, allowing us to establish a close topographical relation between CBF and HbO2. We measured 1CBF by LD flowmetry and tissue HbO2 by a microspectrophotometric method at multiple corresponding locations using a “scanning” technique that employs a computer-controlled micromanipulator. CBF and HbO2 data from predefined locations were correlated to the topographical situation and then arranged in a three-dimensional (3D) image using xyz triplet columns for a mesh and scatter plot. The quantitative topography of the hemodynamic-metabolic interrelation was studied using two experimental models: ischemia/reperfusion (experiment 1), and sinus-vein thrombosis (experiment 2) in rat brain. Using the scanning technique we determined misery and luxury flow (postischemic hyperperfusion) compared with oxygen metabolism in experiment 1 and drainage of desaturated blood via collateral pathways, although detected as flow, in experiment 2. The technique, including the scanning procedure, is useful for showing sequential changes of the hemodynamic-metabolic interrelations of the brain cortex. The technique could reveal phenomena previously undetected by traditional techniques.