6533b7d6fe1ef96bd1265e04

RESEARCH PRODUCT

Denaturation via Surfactants Changes Composition of Protein Corona

Volker MailänderVolker MailänderJulius MüllerJulius MüllerPhilipp RohneClaudia Koch-brandtSvenja MorsbachJohanna SimonKatharina Landfester

subject

Protein Denaturationendocrine systemPolymers and PlasticsNanoparticleBioengineeringProtein Corona02 engineering and technology010402 general chemistry01 natural sciencesBiomaterialsSurface-Active AgentsProtein structurePulmonary surfactantMaterials ChemistryDenaturation (biochemistry)ClusterinbiologyCetrimoniumChemistry021001 nanoscience & nanotechnology0104 chemical sciencesDrug deliverybiology.proteinBiophysicsProtein CoronaNanocarriers0210 nano-technology

description

The use of nanocarriers as drug delivery vehicles brings them into contact with blood plasma proteins. Polymeric nanocarriers require some sort of surfactant to ensure colloidal stability. Formation of the protein corona is therefore determined not only by the intrinsic properties of the nanocarrier itself but also by the accompanying surfactant. Although it is well-known that surfactants have an impact on protein structure, only few studies were conducted on the specific effect of surfactants on the composition of protein corona of nanocarriers. Therefore, we analyzed the composition of the protein corona on "stealth" nanoparticles with additional surfactant (cetyltrimethylammonium chloride, CTMA-Cl) after plasma incubation. Additional CTMA-Cl led to an enrichment of apolipoprotein-A1 and vitronectin in the corona, while less clusterin could be found. Further, the structural stability of apolipoprotein-A1 and clusterin was monitored for a wide range of CTMA-Cl concentrations. Clusterin turned out to be more sensitive to CTMA-Cl, with denaturation occurring at lower concentrations.

yearjournalcountryeditionlanguage
2018-04-16Biomacromolecules
https://doi.org/10.1021/acs.biomac.8b00278