Uptake of silica covered Quantum Dots into living cells: Long term vitality and morphology study on hyaluronic acid biomaterials

6533b7d6fe1ef96bd12665cd

RESEARCH PRODUCT

Uptake of silica covered Quantum Dots into living cells: Long term vitality and morphology study on hyaluronic acid biomaterials

Michele D'amico Benoit Dubertret Gaetano Giammona Maurizio Leone Fabio Salvatore Palumbo Giovanna Pitarresi Valeria Militello Calogero Fiorica

subject

Quantum Dot Nanoparticle Biocompatible Materials 02 engineering and technology 01 natural sciences chemistry.chemical_compound Nanoparticle Labelling Hyaluronic acid Fluorescence microscope Long term cell staining Biocompatible Material Silicon Dioxide 021001 nanoscience & nanotechnology Mechanics of Materials Self-healing hydrogels Materials Science (all)0210 nano-technology Silica Quantum Dot Materials science Fluorescence confocal microscopy Cell Survival Silicon dioxide Chondrocyte bovine cell Hyaluronic acid Confocal Bioengineering Nanotechnology Condensed Matter Physic 010402 general chemistry Biomaterials Chondrocytes Quantum Dots Animals Mechanics of Material Cell Shape Silica Quantum Dots Animal Mechanical Engineering technology industry and agriculture Chondrocyte equipment and supplies Settore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)0104 chemical sciences Microscopy Fluorescence chemistry Settore CHIM/09 - Farmaceutico Tecnologico Applicativo Quantum dot Biophysics Nanoparticles Surface modification Cattle

description

Quantum Dots (QDs) are promising very bright and stable fluorescent probes for optical studies in the biological field but water solubility and possible metal bio-contamination need to be addressed. In this work, a simple silica-QD hybrid system is prepared and the uptake in bovine chondrocytes living cells without any functionalization of the external protective silica shield is demonstrated. Moreover, long term treated cells vitality (up to 14 days) and the transfer of silica-QDs to the next cell generations are here reported. Confocal fluorescence microscopy was also used to determine the morphology of the so labelled cells and the relative silica-QDs distribution. Finally, we employ silica-QD stained chondrocytes to characterize, as proof of concept, hydrogels obtained from an amphiphilic derivative of hyaluronic acid (HA-EDA-C 18) functionalized with different amounts of the RGD peptide.

year	journal	country	edition	language
2015-11-25	Materials Science and Engineering: C

https://doi.org/10.1016/j.msec.2016.04.082