6533b7d6fe1ef96bd126665a
RESEARCH PRODUCT
Inhibition of lactate export by quercetin acidifies rat glial cells in vitro
Oliver KempskiBarbara KempskiChristopher Volksubject
Lactate transportIntracellular pHBiologychemistry.chemical_compoundTumor Cells CulturedmedicineAnimalsheterocyclic compoundsLactic AcidFluorescent DyesGeneral NeuroscienceBiological TransportHydrogen-Ion ConcentrationFluoresceinsIn vitroRatsLactic acidmedicine.anatomical_structurechemistryBiochemistryCell cultureNeurogliaQuercetinQuercetinAcidsNeurogliaIntracellulardescription
The relationship between glial lactate release and glial intracellular pH (pH i) regulation is studied using C6 glioma cells and rat astrocytes in vitro, and the lactate transport inhibitors quercetin and a-cyano-4-hydroxycinnamate (CHC). pHi is measured using 2′,7′bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF). The results show that lactate release is mediated partly by a specific lactate transport system inhibitable by quercetin (50 mM), but not by CHC (5 mM). Inhibition by quercetin results in a significant 3‐4-fold increase of intracellular lactate and a decrease of intracellular pH to 6.9. A participation of quercetin-inhibitable lactate transport in glial pHi-regulation is suggested by the observation that pH i-homeostasis after acidification by diffusion of undissociated lactic acid into the cell is inhibited by quercetin. The existence of a system controlling lactate release in glial cells may also reflect a function of astrocytes to supply neurons with lactate. © 1997 Elsevier Science Ireland Ltd.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-02-21 | Neuroscience Letters |