6533b7d7fe1ef96bd126792f
RESEARCH PRODUCT
The active center of superoxide dismutase from Propionibacterium shermanii
J. HüttermannO. IakovlevaR. KapplBeate MeierFritz G. ParakT. Rimkesubject
biologyExtended X-ray absorption fine structureChemistryPropionibacteriumGeneral Physics and Astronomybiology.organism_classificationSpectral linelaw.inventionActive centerSuperoxide dismutaseCrystallographylawmedicinebiology.proteinFerricMoleculeElectron paramagnetic resonancemedicine.drugdescription
A self-consistent description of the EPR spectra and of the Mossbauer spectra of the natural superoxide dismutase from Propionibacterium shermanii with ferric iron as an active centre is presented. The spectra were measured at pH 6.5, 7.8 and 9.4. The theoretical approach is based on the use of the complete crystal field Hamiltonian for the high-spin ferric complexes with due regard for the terms of the fourth power of the electronic spin. It is shown that a SOD molecule can exist in two conformations. The low-pH conformation has predominantly trigonal symmetry, while the high-pH conformation has the symmetry close to the «extreme rhombic». This interpretation is in full agreement with EXAFS structural data.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-02-01 | Il Nuovo Cimento D |