6533b7d7fe1ef96bd12679f5
RESEARCH PRODUCT
Molecular markers for systematic identification and population genetics of the invasive Ponto-Caspian freshwater gammarid Dikerogammarus villosus (Crustacea, Amphipoda).
Loïc BollacheRémi WattierJérémie BéguetMaria GaillardMarie-jeanne Perrot-minnotJ.c. Müllersubject
GeneticsMitochondrial DNAAmphipodaEcologybiologyZoologyPopulation geneticsDikerogammarus villosusSingle-nucleotide polymorphismSingle-strand conformation polymorphismbiology.organism_classificationBiochemistrynrDNA-ITS1General Biochemistry Genetics and Molecular BiologymicrosatellitesSSCP[ SDV.EE.ECO ] Life Sciences [q-bio]/Ecology environment/Ecosystemsinvasive speciesCOI[ SDV.GEN.GPO ] Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE]Genetic markerMicrosatelliteComputingMilieux_MISCELLANEOUS[ SDV.BID.SPT ] Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomydescription
The Ponto-Caspian amphipod, Dikerogammarus villosus, is an invasive species of many European rivers. First, we show that size difference of nrDNA ITS1 allows discriminating D. villosus from Dikerogammarus bispinosus, a closely related but morphologically hardly distinguishable species. Second, we present two types of polymorphic markers for D. villosus, three microsatellites and two single nucleotide polymorphisms (SNPs) of mtDNA COI gene, which were scored by polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP). These markers will be very useful in studying population genetics of D. villosus.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-06-01 |