6533b7d7fe1ef96bd1267a31
RESEARCH PRODUCT
Induction of cytochrome P450 and/or detoxication enzymes by various extracts or rosemary: description of specific patterns
M SuschetetHervé GoudonnetJean-marie HeydelM.-h SiessP DebersacMarie-josèphe AmiotYves Artursubject
Male[SDV]Life Sciences [q-bio]ReductaseToxicologychemistry.chemical_compoundCytosol0302 clinical medicineCytochrome P-450 Enzyme System[SDV.IDA]Life Sciences [q-bio]/Food engineeringCYTOCHROME P 450AnticarcinogenComputingMilieux_MISCELLANEOUSchemistry.chemical_classificationGLUTATHIONE S-TRANSFERASE0303 health sciencesbiologyReverse Transcriptase Polymerase Chain ReactionChemistryRosmarinic acidOrgan SizeGeneral Medicine[SDV.IDA] Life Sciences [q-bio]/Food engineeringSpecific Pathogen-Free Organisms[SDV] Life Sciences [q-bio]LiverBiochemistryEnzyme Induction030220 oncology & carcinogenesisMicrosomes Liver[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process EngineeringImmunoblottingChemopreventiondigestive system03 medical and health sciencesAnimals[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process EngineeringRNA MessengerRats Wistar030304 developmental biologyLamiaceaePlant ExtractsBody WeightROMARINCytochrome P450GlutathioneUDP-GLUCURONOSYLTRANSFERASENAD(P)H Dehydrogenase (Quinone)RatsEnzymeMicrosomebiology.proteinRATFood Sciencedescription
The ability of rosemary to modulate cytochrome P450 (CYP) and detoxication enzymes in rat liver was evaluated by comparing the effects of dried leaves and leaf extracts with different chemical compositions: essential oil (EO) containing monoterpenes, a dichloromethane extract (DCME) containing phenolic diterpenes and a water-soluble extract (WSE) containing phenolic compounds such as rosmarinic acid and flavonoids. Chemical analyses were done in order to characterize the composition of extracts. Male Wistar rats received the leaves or extracts of rosemary in their diet at 0.5% (w/w) for 2 weeks. The effects of such treatments were evaluated for CYP (1A, 2B, 2E1), glutathione S-transferase (GST), NAD(P)H: quinone reductase (QR) and UDP-glucuronosyltransferase (UGT) activities and on protein levels (immunoblot analyses). Expression of specific UGT isoforms (mRNA semi-quantification by RT-PCR) was measured. Our study reports that EO selectively induced CYP, particularly CYP2B. WSE enhanced both CYP and detoxication enzymes. DCME acted as a monofunctional inducer, inducing GST, QR and UGT, in particular UGT1A6. Considering the specific pattern of induction obtained with DCME and WSE treatment, it should be relevant to evaluate the chemopreventive potency of these extracts on carcinogenesis in animal models.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2001-01-01 |