6533b7d7fe1ef96bd1268461

RESEARCH PRODUCT

Flow Cytometry and Karyotype Analysis ofD. melanogasterEye Disc Cells

Davide CoronaCollesano M

subject

Geneticsbiologymedicine.diagnostic_testKaryotypeEyeFlow Cytometrybiology.organism_classificationTrypsinPhenotypeChromosomesFlow cytometryCell biologyDrosophila melanogasterKaryotypingLarvaInsect SciencemedicineMelanogasterAnimalsDrosophila melanogasterMitosisCell cycle Drosophila Eye-antennal disc Flow cytometry Mitotic chromosomemedicine.drug

description

The developing Drosophila eye-antennal disc is a particularly suited system for the genetic and cellular studies of complex biological processes. Methods to analyze Drosophila eye discs by flow cytometry are mainly based on the dissociation of tissues with trypsin. Dissociation operated by trypsin is very effective, though it causes a lot of stress to live cells often compromising the use of treated cells for further analyses. Here, we report a method to produce dissociated eye-disc cells that retain cell-membrane markers and that can be used for flow cytometry and cytological analysis of mitotic chromosomes. The method described is a great complementing tool for the cellular characterization of phenotypes resulting from classic clonal and miss-expression approaches in the Drosophila eye. DISTRIBUTE. © 2007 Landes Bioscience.

yearjournalcountryeditionlanguage
2008-09-30Fly
https://doi.org/10.4161/fly.4766