LPS injection reprograms the expression and the 3′ UTR of a CAP gene by alternative polyadenylation and the formation of a GAIT element in Ciona intestinalis

6533b7d7fe1ef96bd1268519

RESEARCH PRODUCT

LPS injection reprograms the expression and the 3′ UTR of a CAP gene by alternative polyadenylation and the formation of a GAIT element in Ciona intestinalis

Paolo Colombo Maria Antonietta Sanfratello Angela Bonura Valeria Longo Daniela Parrinello Matteo Cammarata Aiti Vizzini

subject

Lipopolysaccharides 0301 basic medicine Gene isoform Untranslated region Ciona intestinalisCAP proteinPolyadenylationGAIT element LPS Polyadenylation Immunology Settore BIO/05 - Zoologia Biology Polyadenylation Polymerase Chain Reaction 03 medical and health sciences Exon Gene expression Animals Ciona intestinalis Amino Acid Sequence Regulatory Elements Transcriptional 3' Untranslated Regions Molecular Biology Gene In Situ Hybridization Genetics Base Sequence Three prime untranslated region Gene Expression Profiling biology.organism_classification Ciona intestinalis 030104 developmental biology Gene Expression Regulation RNA Cap-Binding Proteins Transcriptome Sequence Alignment

description

The diversification of cellular functions is one of the major characteristics of multicellular organisms which allow cells to modulate their gene expression, leading to the formation of transcripts and proteins with different functions and concentrations in response to different stimuli. CAP genes represent a widespread family of proteins belonging to the cysteine-rich secretory protein, antigen 5 and pathogenesis-related 1 superfamily which, it has been proposed, play key roles in the infection process and the modulation of immune responses in host animals. The ascidian Ciona intestinalis represents a group of proto-chordates with an exclusively innate immune system that has been widely studied in the field of comparative and developmental immunology. Using this biological system, we describe the identification of a novel APA mechanism by which an intronic polyadenylation signal is activated by LPS injection, leading to the formation of a shorter CAP mRNA capable of expressing the first CAP exon plus 19 amino acid residues whose sequence is contained within the first intron of the annotated gene. Furthermore, such an APA event causes the expression of a translational controlling cis-acting GAIT element which is not present in the previously isolated CAP isoform and identified in the 3'-UTR of other immune-related genes, suggesting an intriguing scenario in which both transcriptional and post-transcriptional control mechanisms are involved in the activation of the CAP gene during inflammatory response in C. intestinalis.

year	journal	country	edition	language
2016-09-01	Molecular Immunology

https://doi.org/10.1016/j.molimm.2016.08.004