6533b7d7fe1ef96bd126861e
RESEARCH PRODUCT
Identification of human rotavirus strains with the P[14] genotype by PCR
Cristina AlaimoDaniela PalermoSerenella AristaAntonio CascioEsmeralda Vizzisubject
Microbiology (medical)GeneticsRotavirusReoviridaevirus diseasesGenome ViralBiologymedicine.disease_causebiology.organism_classificationVirologyPolymerase Chain ReactionViruslaw.inventionCapsidCapsidlawRotavirusVirologyGenotypemedicineHumansTypingPolymerase chain reactionSouthern blotROTAVIRUS GASTROENTERITISdescription
ABSTRACT A seminested PCR typing assay has been extended to identify rotavirus strains with the P[14] genotype. The specificity of the method was confirmed by Southern hybridization and by restriction analysis with the enzyme Alu I. One out of four human rotavirus (HRV) strains with unusual subgroup-electropherotype linkage but none out of 50 HRV strains with usual linkage was typed as P[14].
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-01-01 |