6533b7d7fe1ef96bd1268638
RESEARCH PRODUCT
Determination of critical eluent composition for polyethylenglycols using on-line liquid chromatography-Fourier transform infrared spectrometry.
Guillermo QuintásSalvador GarriguesM. De La GuardiaJulia Kuligowskisubject
ChromatographyChemistryAnalytical chemistryInfrared spectroscopyBiochemistryFourier transform spectroscopyAnalytical ChemistryMatrix (chemical analysis)AbsorbanceColumn chromatographyPartial least squares regressionEnvironmental ChemistryBackground Correction MethodFourier transform infrared spectroscopySpectroscopydescription
In this work, it has been extended to methanol:water mobile phases, the use of a background correction method for on-line LC-FTIR measurements named Univariate background correction based on the use of a reference spectra matrix (UBC-RSM) and absorbance ratios. It permits to overcome the problem related to spectral changes occurring during the gradient elution, which in the past limited the on-line coupling of LC and FTIR to isocratic elutions. The combined use of the aforementioned background correction technique in on-line isocratic and gradient LC-FTIR, and partial least squares (PLS) has been applied for the search of the critical conditions for polymers. Polyethylenglycol (PEG) has been used as a model example and results found fitted well with previously published ones.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-08-01 |