6533b7d7fe1ef96bd1268650

RESEARCH PRODUCT

Advanced strategy to exploit wine-making waste by manufacturing antioxidant and prebiotic fibre-enriched vesicles for intestinal health.

Maria Letizia MancaAndrés MoyaXavier Fernàndez-busquetsCarla CaddeoMohamad AllawVicente Pérez-brocalMaria ManconiMaría Del Carmen Recio

subject

Dietary FiberAntioxidantmedicine.medical_treatmentWine02 engineering and technologyGut flora01 natural sciencesAntioxidantsMiceColloid and Surface ChemistryPhospholipid vesiclesFood scienceMice Inbred BALB CSoluble fibre010304 chemical physicsbiologyChemistryVesiclefood and beveragesSurfaces and InterfacesGeneral Medicine021001 nanoscience & nanotechnologyGrape pomaceIntestinal cellsIntestinesHomogeneousFemale0210 nano-technologyBiotechnologyPhospholipid vesiclesCell SurvivalSurface PropertiesGut microbiotaIn vivo studiesAntioxidant activity0103 physical sciencesmedicineAnimalsHumansPrebiotic activityPhysical and Theoretical ChemistryParticle SizeWineWaste ProductsPrebioticfungibiology.organism_classificationGastrointestinal MicrobiomeOxidative StressPrebioticsNutriosomesCaco-2 Cells

description

Grape extract-loaded fibre-enriched vesicles, nutriosomes, were prepared by combining antioxidant extracts obtained from grape pomaces and a prebiotic, soluble fibre (Nutriose®FM06). The nutriosomes were small in size (from ∼140 to 260 nm), homogeneous (polydispersity index < 0.2) and highly negative (∼ −79 mV). The vesicles were highly stable during 12 months of storage at 25 °C. When diluted with warmed (37 °C) acidic medium (pH 1.2) of high ionic strength, the vesicles only displayed an increase of the mean diameter and a low release of the extract, which were dependent on Nutriose concentration. The formulations were highly biocompatible and able to protect intestinal cells (Caco-2) from oxidative stress damage. In vivo results underlined that the composition of mouse microbiota was not affected by the vesicular formulations. Overall results support the potential application of grape nutriosomes as an alternative strategy for the protection of the intestinal tract.

yearjournalcountryeditionlanguage
2020-01-01Colloids and surfaces. B, Biointerfaces
10.1016/j.colsurfb.2020.111146https://pubmed.ncbi.nlm.nih.gov/32485579