6533b7d7fe1ef96bd126866a

RESEARCH PRODUCT

Adhesion, growth and cytoskeletal characteristics of 8701-BC breast carcinoma cells cultured in the presence of type V collagen

Claudio LuparelloSalvatore MinafraRosaria SchillaciIda Pucci-minafra

subject

Pathologymedicine.medical_specialtyCell growthBreast NeoplasmsAdhesionBiologyMolecular biologyIn vitroExtracellular matrixCytoskeletal ProteinsCarcinoma Intraductal NoninfiltratingOncologyCell cultureCell AdhesionTumor Cells CulturedmedicineHumansNeoplastic cellCollagenCytoskeletonBreast carcinomaCell Division

description

Type V collagen is one of the minor components of the extracellular matrix (ECM) whose content is increased in cases of ductal infiltrating carcinomas of the breast. In order to clarify its biological role, we have investigated the effect of this molecule, both as substrate and as soluble factor, on the behaviour of a breast carcinoma cell line (8701-BC) grown in vitro. Cell-collagen adhesion was monitored for 24 h from plating in the absence or presence of serum. The influence of type V collagen on cell growth was followed during 9 days of culture, and the actin-vinculin arrangement was studied by simultaneous fluorescent immuno-staining. The results indicate that type V collagen is not a permissive substrate for neoplastic cell proliferation and dissemination in vitro.

yearjournalcountryeditionlanguage
1990-03-01European Journal of Cancer and Clinical Oncology
https://doi.org/10.1016/0277-5379(90)90217-h