6533b7d7fe1ef96bd126868b

RESEARCH PRODUCT

Isolation and characterization of cold-shock domain protein genes, Oryzias latipes Y-box protein 2 ( OlaYP2 ) and Fugu rubripes Y-box protein 1 ( FruYP1 ), in medakafish and pufferfish

Enchshargal Zend-ajuschThomas HaafZhihong ShanManfred SchartlGötz LütjensCarola BurgtorfUte Hornung

subject

MaleDNA ComplementarySequence analysisOryziasMolecular Sequence DataProtein domainOryziasGene ExpressionBiologyGeneticsAnimalsAmino Acid SequenceIn Situ Hybridization FluorescencePhylogenyComparative genomicsGeneticsBase SequenceSequence Homology Amino AcidFugufungiChromosome MappingSequence Analysis DNAGeneral MedicineY box binding protein 1Cold-shock domainbiology.organism_classificationTakifuguFemaleSequence AlignmentTranscription FactorsBinding domain

description

The Y-box protein (YP) family shares a nucleic acid binding domain, called cold-shock domain, that has been evolutionarily highly conserved from bacteria to human. The different YPs identified so far in vertebrates are thought to function as transcriptional activators, transcriptional repressors and/or translational repressors. Medakafish and pufferfish are very suitable vertebrate models for the study of developmental genetics and comparative genomics, respectively. Here we report the isolation of two teleost YP genes, medakafish Oryzias latipes (Ola)YP2 and Fugu rubripes (Fru)YP1, which are expressed in multiple tissues. Phylogenetic analysis demonstrated that OlaYP2 and FruYP1 belong to different subclasses of the cold-shock domain protein genes. Future studies in suitable model systems, like the medaka for developmental biology and Fugu for evolutionary genomics, are expected to contribute to our understanding of YPs.

yearjournalcountryeditionlanguage
2002-10-18Gene
https://doi.org/10.1016/s0378-1119(02)00838-7