6533b7d7fe1ef96bd1268c92
RESEARCH PRODUCT
Micellar Liquid Chromatography: Fundamentals
Samuel Carda-brochMaria Jose Ruiz-angelMaría Celia García-alvarez-coquesubject
Aqueous solutionColumn chromatographyAqueous normal-phase chromatographyChemistryMicellar liquid chromatographyHydrophilic interaction chromatographytechnology industry and agricultureAnalytical chemistrylipids (amino acids peptides and proteins)macromolecular substancesReversed-phase chromatographyMicelleMicellar electrokinetic chromatographydescription
The reversed phase liquid chromatography (RPLC) mode with surfactant above the critical micellar concentration (CMC) has been called micellar liquid chromatography (MLC). In pure micellar systems, the retention behavior is explained by considering three phases or environments: surfactant-modified stationary phase, bulk aqueous solvent, and micellar pseudo-phase. Surfactant adsorption on the porous RPLC packing affects chromatographic retention, owing to the change of diverse surface properties of the stationary phase. In pure micellar systems, the retention behavior is explained by considering three phases or environments: surfactant-modified stationary phase, bulk aqueous solvent, and micellar pseudo-phase. In pure micellar mobile phases, water-insoluble nonpolar solutes or species strongly bound to surfactant monomers do not participate significantly in the aqueous environment of the three-phase partitioning scheme. Some of the solutions proposed along the years to overcome these limitations have favored the potential of the technique, but they have not resulted in an extensive use. Keywords: bulk aqueous solvent; micellar liquid chromatography; micelle breakdown; retention behavior; reversed phase liquid chromatography; surfactant-modified stationary phase
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2015-11-25 |