6533b7d7fe1ef96bd1268dc7
RESEARCH PRODUCT
Formation of Levan from Raffinose by Levansucrase ofZymomonas mobilis
Lilija AuzinaO. MutereArmands VigantsPeteris ZikmanisInara Andersonesubject
Environmental EngineeringSucrosebiologyMolecular massStereochemistryIntrinsic viscosityLevansucraseBioengineeringFructosebiology.organism_classificationZymomonas mobilischemistry.chemical_compoundchemistryBiochemistryRaffinoseMelibioseBiotechnologydescription
Levansucrase (EC 2.4.1.10.) of Zymomonas mobilis 113S can perform the polymerisation of fructose moiety from raffinose to levan concomitantly with a release of non-catabolised melibiose into the medium. The kinetic parameters of the levansucrase-catalysed reaction provide even higher reaction velocities on raffinose as compared to sucrose, particularly at low substrate concentrations. A decreased value in the number of the average molecular mass (Mn = 1693 kDa), an increased intrinsic viscosity (η = 49.47 cm3/g), and a diminished Huggin's constant (K' = 0.67) are intrinsic to the levan synthesis from raffinose, indicating certain structural peculiarities compared to a polysaccharide obtained from sucrose (Mn = 1851 kDa, [η] = 42.47 cm3/g, K' = 1.21).
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2004-02-05 | Engineering in Life Sciences |