6533b7d8fe1ef96bd126972c

RESEARCH PRODUCT

Immunological Methods for Analysis of Recombinant Proteins

Kaspars TarsIrina Sominskaya

subject

Transformation (genetics)Cloned genesbiologylawPolyclonal antibodiesImmunoscreeningMonoclonalProtein primary structureRecombinant DNAbiology.proteinComputational biologyDNA sequencinglaw.invention

description

We want to introduce researchers to techniques that help to solve some problems in the work of the molecular biologist. After transformation of recombinant DNA in E. coli cells many clones are usually produced, and the same situation appears if recombinant DNA expression libraries are available. Furthermore, if appropriate monoclonal or polyclonal antibodies are available, the method of immunoscreening of colonies for direct immunological detection of translational products of cloned genes can be used. Selected clones could be chosen for further studies such as determination of their primary structure by DNA sequencing and for characterization of an appropriate expressed protein.

yearjournalcountryeditionlanguage
1998-01-01
https://doi.org/10.1007/978-3-642-71965-3_7